Guat Lay, Caroline Lee
Postgraduate:
Main Appointment:
Joint Appointments:
Research Fields:
Research Areas:
Research Fields:
- STEMM – Science, Technology, Engineering, Mathematics, Medical Sciences
Research Keywords:
- Population And Pharmacogenetics
- Non-coding RNA Including LncRNA, CircRNA And MiRNA
- Big Data Genomics Including Machine Learning / Artificial Intelligence
- Hepatocellular Carcinoma Or Liver Cancer
- Drug Response
Current Appointments:
Brief Description of Research:
• Precision Medicine
o Machine Learning to identify pfSNPs (potentially functional polymorphisms) biomarkers for the prediction of drug response or side effects.
o Predicting population differences in drug response based on population differentiated, potentially functional (pdpfSNPs)
• Liver Cancer / Hepatocellular Carcinoma (HCC)
o FAT10 as target for Inflammation-Associated Cancers
o Characterization of the role of HBV and HBx gene in HCC
o Non-coding RNAs (ncRNA including circRNAs, lncRNAs and miRNAs) in Cancer
Using Artificial Intelligence (AI) to identify signatures of ncRNAs / clinical / demographic characteristics as prognostic biomarkers
Identify key nodal ncRNAs in clinically-relevant network of ncRNA-mRNAs in cancer pathways modulating prognosis as therapeutic targets
Total Number of Publications:
Five Representative Publications:
1. Josephine Yu Yan Yap, Laura Shih Hui Goh, Ashley Jun Wei Lim, Samuel S. Chong, Lee Jin Lim and Caroline G. Lee. Machine Learning Identifies a Signature of Nine Exosomal RNAs That Predicts Hepatocellular Carcinoma (2023). Cancers 2023, 15, 3749. https://doi.org/10.3390/cancers15143749.
2. Ashley Lim Jun Wei; Tera Tyniana; Lee Jin Lim; Justina Wei Lynn Tan; Ee Tzun Koh; Samuel Chong; Chiea Cheun Khor; Khai Pang Leong; Caroline Lee (2023). Robust SNP-based prediction of Rheumatoid Arthritis through Machine-Learning-Optimized Polygenic Risk Score. Journal of Translational Medicine. J Transl Med 21, 92 (2023). https://doi.org/10.1186/s12967-023-03939-5.
3. Sock Hoai Chan, Yasmin Bylstra, Jing Xian Teo, Jyn Ling Kuan, Nicolas Bertin, Mar Gonzalez-Porta, Maxime Hebrard, Roberto Tirado-Magallanes, Joanna Hui Juan Tan, Justin Jeyakani, Zhihui Li, Jin Fang Chai, Yap Seng Chong, Sonia Davila, Liuh Ling Goh, Eng Sing Lee, Eleanor Wong, Tien Yin Wong; SG10K_Health Consortium (…Caroline G. Lee…); Shyam Prabhakar, Jianjun Liu, Ching-Yu Cheng, Birgit Eisenhaber, Neerja Karnani, Khai Pang Leong, Xueling Sim, Khung Keong Yeo, John C Chambers, E-Shyong Tai, Patrick Tan, Saumya S Jamuar, Joanne Ngeow, Weng Khong Lim. Analysis of clinically relevant variants from ancestrally diverse Asian genomes. Nat Commun. 2022 Nov 5;13(1):6694. doi: 10.1038/s41467-022-34116-9.
4. Ashley J.W. Lim, Lee Jin Lim, Brandon N. S. Ooi, Ee Tzun Koh, Justina Wei Lynn Tan, TTSH RA Study Group, Samuel S. Chong, Chiea Chuen Khor, Lisa Tucker-Kellogg, Khai Pang Leong, Caroline G. Lee*. (2022) Functional coding haplotypes and machine-learning feature elimination identifies predictors of Methotrexate Response in Rheumatoid Arthritis patients. EBioMedicine https://doi.org/10.1016/j.ebiom.2021.103800 (https://www.thelancet.com/pdfs/journals/ebiom/PIIS2352-3964(21)00594-6.pdf)
5. Lee Jin Lim†, Ashley J.W. Lim†, Brandon N. S. Ooi , Justina Wei Lynn Tan, Ee Tzun Koh, TTSH Rheumatoid Arthritis Study Group, Samuel S. Chong, Chiea Chuen Khor, Lisa Tucker-Kellogg, Caroline G. Lee*, Khai Pang Leong*. (2022) Machine Learning using Genetic and Clinical Data Identifies a Signature that Robustly Predicts Methotrexate Response in Rheumatoid Arthritis. Rheumatology https://doi.org/10.1093/rheumatology/keac032 *co-corresponding authors.
6. Brandon N. S. Ooi, Ashley J. W. Lim, Samuel S. Chong, and Caroline G. L. Lee. Using Genome Wide Studies to Generate and Test Hypotheses that Provide Mechanistic Details of How Synonymous Codons Affect Protein Structure and Function: Functional SNPs in the Age of Precision Medicine. (2022) Chapter 8. Pg 171-183 In 'Single Nucleotide Polymorphisms. Human Variation and a Coming Revolution in Biology and Medicine. Eds: Zuben E. Sauna and Chava Kimchi-Sarfaty (Springer).
7. Lee Jin Lim, Ashley Jun Wei Lim, Kevin K. Fong, Caroline G. Lee. Sentiments regarding COVID-19 Vaccination among Graduate Students in Singapore. Vaccines (2021) 9:1141-1156. https://doi.org/10.3390/vaccines9101141
8. Jian Han , Thomas Thurnherr , Alexander Y.F. Chung , Brian K.P. Goh , Pierce K.H. Chow , Chung Yip Chan , Peng Chung Cheow , Ser Yee Lee , Tony K.H. Lim , Samuel S . Chong , London L.P.J. Ooi , Caroline G Lee *. Clinicopathological-associated regulatory network of deregulated circRNAs in hepatocellular carcinoma. Cancers (2021) 13, 2772. https://doi.org/10.3390/cancers13112772.
9. Brandon N. S. Ooi, Raechell, Ariel F. Ying, Yong Zher Koh, Yu Jin, Sherman W. L. Yee, Justin H. S. Lee, Samuel S. Chong, Jack W. C. Tan, Jianjun Liu, Caroline G. Lee* and Chester L. Drum*. Robust performance of potentially functional SNPs in machine learning models for the prediction of atorvastatin-induced myalgia. Frontiers in Pharmacology (2021) 12:605764. doi: 10.3389/fphar.2021.605764
10. Lee Jin Lim, Lay Hiang Ling, Yu Pei Neo, Alexander Y.F. Chung, Brian K.P. Goh, Pierce K.H. Chow, Chung Yip Chan, Peng Chung Cheow, Ser Yee Lee, Tony K.H. Lim, Samuel S. Chong, London L.P.J.Ooi and Caroline G. Lee*. Highly deregulated lncRNA LOC is associated with overall worse prognosis in Hepatocellular Carcinoma patients. Journal of Cancer (2021) 12(11):3098-3113. doi:10.7150/jca.56340
11. Lee Jin Lim, Yu Jin, Henry Yang, Alexander Y.F. Chung, Brian K.P. Goh, Pierce K.H. Chow, Chung Yip Chan, William K. Blanks, Peng Chung Cheow, Ser Yee Lee, Tony K.H. Lim, Samuel S. Chong, London L.P.J.Ooi and Caroline G. Lee. Network of clinically-relevant lncRNAs-mRNAs associated with prognosis of Hepatocellular Carcinoma patients. Scientific Reports (2020) 10:11124
12. Maulana Bachtiar, Brandon Nick Sern Ooi, Jingbo Wang, Jin Yu, Tin Wee Tan, Samuel S. Chong, and Caroline G. L. Lee*. (2019) Towards Precision Medicine: Interrogating the Human Genome to Identify Drug Pathways Associated with Potentially Functional, Population-Differentiated Polymorphisms. The Pharmacogenomics Journal doi.org/10. 1038/541397-019–0096-y.
13. Lee Jin Lim, Samuel Y.S. Wong, Feiyang Huang, Sheng Lim, Samuel Chong, London Lucien Ooi, Oi Lian Kon and Caroline G. Lee*. Roles and Regulation of Long non-coding RNAs in Hepatocellular Carcinoma. Cancer Research DOI: 10.1158/0008-5472.CAN-19-0255 (2019).
14. Lee Wai Yeow, Maulana Bachtiar, Chan Siew Choo, Cheryl and Caroline G Lee*. Comprehensive Review of Hepatitis B Virus-associated Hepatocellular Carcinoma Research through Text Mining and Big Data Analytics. Biol Rev Camb Philos Soc. 2019 Apr;94(2):353-367. doi: 10.1111/brv.12457. Epub 2018
15. Cheryl CHAN, Thomas THURNHERR, Jingbo WANG, Xavier GALLART-PALAU, Siu Kwan SZE, Steve ROZEN and Caroline G. LEE. Global re-wiring of p53 transcription regulation by the hepatitis B virus X protein. Molecular Oncology 2016 10(8):1183-1195 (2016)
16. Steven Setiawan Theng, Wei Wang, Way Champ Mah, Cheryl Chan, Jingli Zhuo, Yun Gao, Haina Qin, Liangzhong Lim, Samuel S. Chong, Jianxing Song and Caroline G.L. Lee*. Disruption of FAT10-MAD2 binding inhibits Tumor Progression Proc. Natl. Acad. Sci. U.S.A. 111(49):E5282-5291 (2014)
My Research Videos:
Top 5 Publications:
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Journals Published:
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Guat Lay, Caroline Lee
Appointments
Education
Research Areas
- Population And Pharmacogenetics
- Non-coding RNA Including LncRNA, CircRNA And MiRNA
- Big Data Genomics Including Machine Learning / Artificial Intelligence
- Hepatocellular Carcinoma Or Liver Cancer
- Drug Response
Research Description
• Precision Medicine
o Machine Learning to identify pfSNPs (potentially functional polymorphisms) biomarkers for the prediction of drug response or side effects.
o Predicting population differences in drug response based on population differentiated, potentially functional (pdpfSNPs)
• Liver Cancer / Hepatocellular Carcinoma (HCC)
o FAT10 as target for Inflammation-Associated Cancers
o Characterization of the role of HBV and HBx gene in HCC
o Non-coding RNAs (ncRNA including circRNAs, lncRNAs and miRNAs) in Cancer
Using Artificial Intelligence (AI) to identify signatures of ncRNAs / clinical / demographic characteristics as prognostic biomarkers
Identify key nodal ncRNAs in clinically-relevant network of ncRNA-mRNAs in cancer pathways modulating prognosis as therapeutic targets
Research Videos
Selected Publications
1. Josephine Yu Yan Yap, Laura Shih Hui Goh, Ashley Jun Wei Lim, Samuel S. Chong, Lee Jin Lim and Caroline G. Lee. Machine Learning Identifies a Signature of Nine Exosomal RNAs That Predicts Hepatocellular Carcinoma (2023). Cancers 2023, 15, 3749. https://doi.org/10.3390/cancers15143749.
2. Ashley Lim Jun Wei; Tera Tyniana; Lee Jin Lim; Justina Wei Lynn Tan; Ee Tzun Koh; Samuel Chong; Chiea Cheun Khor; Khai Pang Leong; Caroline Lee (2023). Robust SNP-based prediction of Rheumatoid Arthritis through Machine-Learning-Optimized Polygenic Risk Score. Journal of Translational Medicine. J Transl Med 21, 92 (2023). https://doi.org/10.1186/s12967-023-03939-5.
3. Sock Hoai Chan, Yasmin Bylstra, Jing Xian Teo, Jyn Ling Kuan, Nicolas Bertin, Mar Gonzalez-Porta, Maxime Hebrard, Roberto Tirado-Magallanes, Joanna Hui Juan Tan, Justin Jeyakani, Zhihui Li, Jin Fang Chai, Yap Seng Chong, Sonia Davila, Liuh Ling Goh, Eng Sing Lee, Eleanor Wong, Tien Yin Wong; SG10K_Health Consortium (…Caroline G. Lee…); Shyam Prabhakar, Jianjun Liu, Ching-Yu Cheng, Birgit Eisenhaber, Neerja Karnani, Khai Pang Leong, Xueling Sim, Khung Keong Yeo, John C Chambers, E-Shyong Tai, Patrick Tan, Saumya S Jamuar, Joanne Ngeow, Weng Khong Lim. Analysis of clinically relevant variants from ancestrally diverse Asian genomes. Nat Commun. 2022 Nov 5;13(1):6694. doi: 10.1038/s41467-022-34116-9.
4. Ashley J.W. Lim, Lee Jin Lim, Brandon N. S. Ooi, Ee Tzun Koh, Justina Wei Lynn Tan, TTSH RA Study Group, Samuel S. Chong, Chiea Chuen Khor, Lisa Tucker-Kellogg, Khai Pang Leong, Caroline G. Lee*. (2022) Functional coding haplotypes and machine-learning feature elimination identifies predictors of Methotrexate Response in Rheumatoid Arthritis patients. EBioMedicine https://doi.org/10.1016/j.ebiom.2021.103800 (https://www.thelancet.com/pdfs/journals/ebiom/PIIS2352-3964(21)00594-6.pdf)
5. Lee Jin Lim†, Ashley J.W. Lim†, Brandon N. S. Ooi , Justina Wei Lynn Tan, Ee Tzun Koh, TTSH Rheumatoid Arthritis Study Group, Samuel S. Chong, Chiea Chuen Khor, Lisa Tucker-Kellogg, Caroline G. Lee*, Khai Pang Leong*. (2022) Machine Learning using Genetic and Clinical Data Identifies a Signature that Robustly Predicts Methotrexate Response in Rheumatoid Arthritis. Rheumatology https://doi.org/10.1093/rheumatology/keac032 *co-corresponding authors.
6. Brandon N. S. Ooi, Ashley J. W. Lim, Samuel S. Chong, and Caroline G. L. Lee. Using Genome Wide Studies to Generate and Test Hypotheses that Provide Mechanistic Details of How Synonymous Codons Affect Protein Structure and Function: Functional SNPs in the Age of Precision Medicine. (2022) Chapter 8. Pg 171-183 In 'Single Nucleotide Polymorphisms. Human Variation and a Coming Revolution in Biology and Medicine. Eds: Zuben E. Sauna and Chava Kimchi-Sarfaty (Springer).
7. Lee Jin Lim, Ashley Jun Wei Lim, Kevin K. Fong, Caroline G. Lee. Sentiments regarding COVID-19 Vaccination among Graduate Students in Singapore. Vaccines (2021) 9:1141-1156. https://doi.org/10.3390/vaccines9101141
8. Jian Han , Thomas Thurnherr , Alexander Y.F. Chung , Brian K.P. Goh , Pierce K.H. Chow , Chung Yip Chan , Peng Chung Cheow , Ser Yee Lee , Tony K.H. Lim , Samuel S . Chong , London L.P.J. Ooi , Caroline G Lee *. Clinicopathological-associated regulatory network of deregulated circRNAs in hepatocellular carcinoma. Cancers (2021) 13, 2772. https://doi.org/10.3390/cancers13112772.
9. Brandon N. S. Ooi, Raechell, Ariel F. Ying, Yong Zher Koh, Yu Jin, Sherman W. L. Yee, Justin H. S. Lee, Samuel S. Chong, Jack W. C. Tan, Jianjun Liu, Caroline G. Lee* and Chester L. Drum*. Robust performance of potentially functional SNPs in machine learning models for the prediction of atorvastatin-induced myalgia. Frontiers in Pharmacology (2021) 12:605764. doi: 10.3389/fphar.2021.605764
10. Lee Jin Lim, Lay Hiang Ling, Yu Pei Neo, Alexander Y.F. Chung, Brian K.P. Goh, Pierce K.H. Chow, Chung Yip Chan, Peng Chung Cheow, Ser Yee Lee, Tony K.H. Lim, Samuel S. Chong, London L.P.J.Ooi and Caroline G. Lee*. Highly deregulated lncRNA LOC is associated with overall worse prognosis in Hepatocellular Carcinoma patients. Journal of Cancer (2021) 12(11):3098-3113. doi:10.7150/jca.56340
11. Lee Jin Lim, Yu Jin, Henry Yang, Alexander Y.F. Chung, Brian K.P. Goh, Pierce K.H. Chow, Chung Yip Chan, William K. Blanks, Peng Chung Cheow, Ser Yee Lee, Tony K.H. Lim, Samuel S. Chong, London L.P.J.Ooi and Caroline G. Lee. Network of clinically-relevant lncRNAs-mRNAs associated with prognosis of Hepatocellular Carcinoma patients. Scientific Reports (2020) 10:11124
12. Maulana Bachtiar, Brandon Nick Sern Ooi, Jingbo Wang, Jin Yu, Tin Wee Tan, Samuel S. Chong, and Caroline G. L. Lee*. (2019) Towards Precision Medicine: Interrogating the Human Genome to Identify Drug Pathways Associated with Potentially Functional, Population-Differentiated Polymorphisms. The Pharmacogenomics Journal doi.org/10. 1038/541397-019–0096-y.
13. Lee Jin Lim, Samuel Y.S. Wong, Feiyang Huang, Sheng Lim, Samuel Chong, London Lucien Ooi, Oi Lian Kon and Caroline G. Lee*. Roles and Regulation of Long non-coding RNAs in Hepatocellular Carcinoma. Cancer Research DOI: 10.1158/0008-5472.CAN-19-0255 (2019).
14. Lee Wai Yeow, Maulana Bachtiar, Chan Siew Choo, Cheryl and Caroline G Lee*. Comprehensive Review of Hepatitis B Virus-associated Hepatocellular Carcinoma Research through Text Mining and Big Data Analytics. Biol Rev Camb Philos Soc. 2019 Apr;94(2):353-367. doi: 10.1111/brv.12457. Epub 2018
15. Cheryl CHAN, Thomas THURNHERR, Jingbo WANG, Xavier GALLART-PALAU, Siu Kwan SZE, Steve ROZEN and Caroline G. LEE. Global re-wiring of p53 transcription regulation by the hepatitis B virus X protein. Molecular Oncology 2016 10(8):1183-1195 (2016)
16. Steven Setiawan Theng, Wei Wang, Way Champ Mah, Cheryl Chan, Jingli Zhuo, Yun Gao, Haina Qin, Liangzhong Lim, Samuel S. Chong, Jianxing Song and Caroline G.L. Lee*. Disruption of FAT10-MAD2 binding inhibits Tumor Progression Proc. Natl. Acad. Sci. U.S.A. 111(49):E5282-5291 (2014)